Sanjiv S. Gambhir

6 Hidalgo‐Lozano A, Fernández‐de‐las‐Peñas C, Alonso‐Blanco C, et al. Muscle trigger points and pressure pain hyperalgesia in the shoulder muscles in patients with unilateral shoulder impingement: a blinded, controlled study. Exp Brain Res 2010; 202: 915–25. doi: 10.1007/s00221‐010‐2196‐47 Clausen MB, Bandholm T, Rathleff MS, et al. The Strengthening Exercises in Shoulder Impingement trial (The SExSI‐trial) investigating the effectiveness of a simple add‐on shoulder strengthening exercise programme in patients with long‐lasting subacromial impingement syndrome: Study protocol for a pragmatic, assessor blinded, parallel‐group, randomised, controlled trial. Trials 2018; 19: 154. doi: 10.1186/s13063‐018‐2509‐7

The present invention provides methods for diagnosing lung cancer in a subject comprising (a) generating circulating tumor cell (CTC) data from a blood sample obtained from the subject based on a direct analysis comprising immunofluorescent staining and morphological characteristics of nucleated cells in the sample, wherein CTCs are identified in context of surrounding nucleated cells based on a combination of the immunofluorescent staining and morphological characteristics;(b) obtaining clinical data for the subject;(c) combining the CTC data with the clinical data to diagnose lung cancer in the subject.

Raman spectroscopy provides excellent specificity for in vivo preclinical imaging through a readout of fingerprint-like spectra. To achieve sufficient sensitivity for in vivo Raman imaging, metallic gold nanoparticles larger than 10 nm were employed to amplify Raman signals via surface-enhanced Raman scattering (SERS). However, the inability to excrete such large gold nanoparticles has restricted the translation of Raman imaging. Here we present Raman-active metallic gold supraclusters that are biodegradable and excretable as nanoclusters. Although the small size of the gold nanocluster building blocks compromises the electromagnetic field enhancement effect, the supraclusters exhibit bright and prominent Raman scattering comparable to that of large gold nanoparticle-based SERS nanotags due to high loading of NIR-resonant Raman dyes and much suppressed fluorescence background by metallic …

A device (“the ExoTIC device”) for the isolation of extracellular vesicles from an extracellular vesicle-containing sample in which the sample is flowed through a membrane in a flow chamber to capture and purify the extracellular vesicles on the membrane. The extracellular vesicles may be washed and collected and utilized in any one of a number of ways including, but not limited to, identifying biomarkers of a disease, identifying the presence of a biomarker in a patient to determine whether a patient has a disease, and therapeutically treating existing diseases by re-introducing the extracellular vesicles, potentially modified, back into a body.

Although literature suggests that resistance to TNF inhibitor (TNFi) therapy in patients with ulcerative colitis (UC) is partially linked to immune cell populations in the inflamed region, there is still substantial uncertainty underlying the relevant spatial context. Here, we used the highly multiplexed immunofluorescence imaging technology CODEX to create a publicly browsable tissue atlas of inflammation in 42 tissue regions from 29 patients with UC and 5 healthy individuals. We analyzed 52 biomarkers on 1,710,973 spatially resolved single cells to determine cell types, cell-cell contacts, and cellular neighborhoods. We observed that cellular functional states are associated with cellular neighborhoods. We further observed that a subset of inflammatory cell types and cellular neighborhoods are present in patients with UC with TNFi treatment, potentially indicating resistant niches. Last, we explored applying convolutional …

As published previously, in Figure 7c− e, we had inadvertently and erroneously repeated the same images twice in panels c and e while missing an image for day 12 in panel d. We now include the correct images in the panels. The other components of

In the original version of our paper (https://pubs. acs. org/ doi/10.1021/acsami. 9b20071), Figure 6 f shows the magnified images of H&E stained kidney tissues for both TK− NTR− SP94 and TK− p53− NTR conditions that were inadvertently and erroneously taken from the same TK− p53− NTR slide. The correct images are shown here. Figure 6 a− e) are correct as published previously. There is no change made to the figure legend. This error does not change the fundamental nature or message of our findings.

Surface-enhanced Raman scattering (SERS) has extended its biomedical application to in vivo preclinical imaging. However, the in vivo SERS imaging requires non-clearable large gold nanoparticles, limiting their translation in humans. Here, we address this problem by creating SERS supraparticles composed of small-sized nanoclusters. First, we performed the FDTD simulation of the supraparticle design, and the maximum enhancement factor of 10^6 was achieved. Second, we chemically synthesized bright supraparticles that enabled in vivo Raman imaging of rodent models. Furthermore, the supraparticles were highly excretable, offering great potential for clinical application of in vivo Raman imaging by replacing non-excretable SERS nanotags.