paul klenerman
University of Oxford
H-index: 136
Europe-United Kingdom
Description
paul klenerman, With an exceptional h-index of 136 and a recent h-index of 91 (since 2020), a distinguished researcher at University of Oxford, specializes in the field of Infection and Immunity.
His recent articles reflect a diverse array of research interests and contributions to the field:
Immune responses and clinical outcomes after COVID-19 vaccination in patients with liver disease and liver transplant recipients
Multi-omics analysis reveals COVID-19 vaccine induced attenuation of inflammatory responses during breakthrough disease
MAIT cells play a role in liver tissue repair via growth factor secretion
The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA. 2.86
N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting
IL-5 antagonism reverses priming and activation of eosinophils in severe eosinophilic asthma
Obesity Differs from Diabetes Mellitus in Antibody and T Cell Responses Post COVID-19 Recovery
Gonadal androgens are associated with decreased type I interferon production by plasmacytoid dendritic cells and increased IgG titres to BNT162b2 following co-vaccination with …
Professor Information
University | University of Oxford |
---|---|
Position | ___ |
Citations(all) | 82913 |
Citations(since 2020) | 46440 |
Cited By | 48411 |
hIndex(all) | 136 |
hIndex(since 2020) | 91 |
i10Index(all) | 519 |
i10Index(since 2020) | 374 |
University Profile Page | University of Oxford |
Research & Interests List
Infection and Immunity
Top articles of paul klenerman
Immune responses and clinical outcomes after COVID-19 vaccination in patients with liver disease and liver transplant recipients
Background & AimsComparative assessments of immunogenicity following different COVID-19 vaccines in patients with distinct liver diseases are lacking. SARS-CoV-2-specific T-cell and antibody responses were evaluated longitudinally after one to three vaccine doses, with long-term follow-up for COVID-19-related clinical outcomes.MethodsA total of 849 participants (355 with cirrhosis, 74 with autoimmune hepatitis [AIH], 36 with vascular liver disease [VLD], 257 liver transplant recipients [LTRs] and 127 healthy controls [HCs]) were recruited from four countries. Standardised immune assays were performed pre and post three vaccine doses (V1-3).ResultsIn the total cohort, there were incremental increases in antibody titres after each vaccine dose (p <0.0001). Factors associated with reduced antibody responses were age and LT, whereas heterologous vaccination, prior COVID-19 and mRNA platforms were …
Authors
Sam M Murray,Elisa Pose,Melanie Wittner,Maria-Carlota Londoño,Golda Schaub,Jonathan Cook,Stavros Dimitriadis,Georgina Meacham,Sophie Irwin,Zixiang Lim,Paul Duengelhoef,Martina Sterneck,Ansgar W Lohse,Valeria Perez,Palak Trivedi,Khush Bhandal,Benjamin H Mullish,Pinelopi Manousou,Nicholas M Provine,Emma Avitabile,Miles Carroll,Tom Tipton,Saoirse Healy,Patrizia Burra,Paul Klenerman,Susanna Dunachie,Barbara Kronsteiner,Agnieszka Katarzyna Maciola,Giulia Pasqual,Virginia Hernandez-Gea,Juan Carlos Garcia-Pagan,Pietro Lampertico,Massimo Iavarone,Pere Gines,Marc Lütgehetmann,Julian Schulze Zur Wiesch,Francesco Paolo Russo,Eleanor Barnes,Thomas Marjot,OCTAVE Collaborative Group
Journal
Journal of Hepatology
Published Date
2024/1/1
Multi-omics analysis reveals COVID-19 vaccine induced attenuation of inflammatory responses during breakthrough disease
The immune mechanisms mediating COVID-19 vaccine attenuation of COVID-19 remain undescribed. We conducted comprehensive analyses detailing immune responses to SARS-CoV-2 virus in blood post-vaccination with ChAdOx1 nCoV-19 or a placebo. Samples from randomised placebo-controlled trials (NCT04324606 and NCT04400838) were taken at baseline, onset of COVID-19-like symptoms, and 7 days later, confirming COVID-19 using nucleic amplification test (NAAT test) via real-time PCR (RT-PCR). Serum cytokines were measured with multiplexed immunoassays. The transcriptome was analysed with long, short and small RNA sequencing. We found attenuation of RNA inflammatory signatures in ChAdOx1 nCoV-19 compared with placebo vaccinees and reduced levels of serum proteins associated with COVID-19 severity. KREMEN1, a putative alternative SARS-CoV-2 receptor, was …
Authors
Ruth E Drury,Susana Camara,Irina Chelysheva,Sagida Bibi,Katherine Sanders,Salle Felle,Katherine Emary,Daniel Phillips,Merryn Voysey,Daniela M Ferreira,Paul Klenerman,Sarah C Gilbert,Teresa Lambe,Andrew J Pollard,Daniel O’Connor
Journal
Nature Communications
Published Date
2024/4/22
MAIT cells play a role in liver tissue repair via growth factor secretion
IntroductionMAIT cells (Mucosa-Associated Invariant T cells) are highly enriched in the human liver, representing about 20-50% of intrahepatic CD8+ T cells. These cells express transcriptional signatures associated with tissue repair both in vitro and in vivo, even though the direct mediators of MAIT cell-directed tissue repair in human tissues have not been defined. The aim of our study is to identify such factors and their in vivo relevance, especially in liver injury and regeneration.MethodsPeripheral blood PBMCs and isolated MAIT cells from healthy donors were stimulated via their TCR, with cytokines, or with both stimuli for 72h. The concentration of growth factors in these culture supernatants was quantified using LEGENDplex. MAIT cell supernatants with or without blocking antibodies were used in wound-healing assays with Caco-2 (epithelial cells) and HHL12 (hepatocytes) cell monolayers to functionally …
Authors
K Sayaf,I Tasin,HD Akther,LC Garner,N Ramamurthy,CP Hackstein,FP Russo,P Klenerman
Journal
Digestive and Liver Disease
Published Date
2024/2/1
The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA. 2.86
Under pressure from neutralising antibodies induced by vaccination or infection the SARS-CoV-2 spike gene has become a hotspot for evolutionary change, leading to the failure of all mAbs developed for clinical use. Most potent antibodies bind to the receptor binding domain which has become heavily mutated. Here we study responses to a conserved epitope in sub-domain-1 (SD1) of spike which have become more prominent because of mutational escape from antibodies directed to the receptor binding domain. Some SD1 reactive mAbs show potent and broad neutralization of SARS-CoV-2 variants. We structurally map the dominant SD1 epitope and provide a mechanism of action by blocking interaction with ACE2. Mutations in SD1 have not been sustained to date, but one, E554K, leads to escape from mAbs. This mutation has now emerged in several sublineages including BA.2.86, reflecting selection …
Authors
Daming Zhou,Piyada Supasa,Chang Liu,Aiste Dijokaite-Guraliuc,Helen ME Duyvesteyn,Muneeswaran Selvaraj,Alexander J Mentzer,Raksha Das,Wanwisa Dejnirattisai,Nigel Temperton,Paul Klenerman,Susanna J Dunachie,Elizabeth E Fry,Juthathip Mongkolsapaya,Jingshan Ren,David I Stuart,Gavin R Screaton
Journal
Nature Communications
Published Date
2024/3/28
N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting
In vitro-transcribed (IVT) mRNAs are modalities that can combat human disease, exemplified by their use as vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). IVT mRNAs are transfected into target cells, where they are translated into recombinant protein, and the biological activity or immunogenicity of the encoded protein exerts an intended therapeutic effect,. Modified ribonucleotides are commonly incorporated into therapeutic IVT mRNAs to decrease their innate immunogenicity, –, but their effects on mRNA translation fidelity have not been fully explored. Here we demonstrate that incorporation of N1-methylpseudouridine into mRNA results in +1 ribosomal frameshifting in vitro and that cellular immunity in mice and humans to +1 frameshifted products from BNT162b2 vaccine mRNA translation occurs after vaccination. The +1 ribosome frameshifting observed is probably a consequence …
Authors
Thomas E Mulroney,Tuija Pöyry,Juan Carlos Yam-Puc,Maria Rust,Robert F Harvey,Lajos Kalmar,Emily Horner,Lucy Booth,Alexander P Ferreira,Mark Stoneley,Ritwick Sawarkar,Alexander J Mentzer,Kathryn S Lilley,C Mark Smales,Tobias von der Haar,Lance Turtle,Susanna Dunachie,Paul Klenerman,James ED Thaventhiran,Anne E Willis
Journal
Nature
Published Date
2024/1/4
IL-5 antagonism reverses priming and activation of eosinophils in severe eosinophilic asthma
Eosinophils are key effector cells mediating airway inflammation and exacerbation in patients with severe eosinophilic asthma. They are present in increased numbers and activation states in the airway mucosa and lumen. Interleukin-5 (IL-5) is the key eosinophil growth factor that is thought to play a role in eosinophil priming and activation. However, the mechanism of these effects is still not fully understood. The anti-IL-5 antibody mepolizumab reduces eosinophil counts in the airway modestly but has a large beneficial effect on the frequency of exacerbations of severe eosinophilic asthma, suggesting that reduction in eosinophil priming and activation is of central mechanistic importance. In this study, we used the therapeutic effect of mepolizumab and single-cell ribonucleic acid sequencing to investigate the mechanism of eosinophil priming and activation by IL-5. We demonstrated that IL-5 is a dominant driver of …
Authors
Jian Luo,Wentao Chen,Wei Liu,Shan Jiang,Yuan Ye,Rahul Shrimanker,Gareth Hynes,Paul Klenerman,Ian D Pavord,Luzheng Xue
Journal
Mucosal Immunology
Published Date
2024/3/15
Obesity Differs from Diabetes Mellitus in Antibody and T Cell Responses Post COVID-19 Recovery
Objective:Obesity and type 2 diabetes (DM) are risk factors for severe COVID-19 outcomes, which disproportionately affect South Asian populations. This study aims to investigate the humoral and cellular immune responses to SARS-CoV-2 in adult COVID-19 survivors with obesity and DM in Bangladesh.Methods:In this cross-sectional study, SARS-CoV-2-specific antibody and T cell responses were investigated in 63 healthy and 75 PCR-confirmed COVID-19 recovered individuals in Bangladesh, during the pre-vaccination first wave of the COVID-19 pandemic in 2020.Results:In COVID-19 survivors, SARS-CoV-2 infection induced robust antibody and T cell responses, which correlated with disease severity. After adjusting for age, sex, DM status, disease severity, and time since onset of symptoms, obesity was associated with decreased neutralising antibody titers, and increased SARS-CoV-2 spike-specific IFN-{gamma} response along with increased proliferation and IL-2 production by CD8+ T cells. In contrast, DM was not associated with SARS-CoV-2-specific antibody and T cell responses after adjustment for obesity and other confounders.Conclusions:Obesity is associated with lower neutralising antibody levels and higher T cell responses to SARS-CoV-2 post COVID-19 recovery, while antibody or T cell responses remain unaltered in DM.
Authors
Mohammad Ali,Stephanie Longet,Isabel Neale,Patpong Rongkard,Forhad Uddin Hassan Chowdhury,Jennifer Hill,Anthony Brown,Stephen Laidlaw,Tom Tipton,Ashraful Hoque,Nazia Hassan,Carl-Philipp Hackstein,Sandra Adele,Hossain Delowar Akther,Priyanka Abraham,Shrebash Paul,Md Matiur Rahman,Md Masum Alam,Shamima Parvin,Forhadul Hoque Mollah,Md Mozammel Hoque,Shona C Moore,Subrata K Biswas,Lance Turtle,Thushan I de Silva,Ane Ogbe,John Frater,Eleanor Barnes,Adriana Tomic,Miles W Carroll,Paul Klenerman,Barbara Kronsteiner,Fazle Rabbi Chowdhury,Susanna J Dunachie
Published Date
2023
Gonadal androgens are associated with decreased type I interferon production by plasmacytoid dendritic cells and increased IgG titres to BNT162b2 following co-vaccination with …
mRNA vaccine technologies introduced following the SARS-CoV-2 pandemic have highlighted the need to better understand the interaction of adjuvants and the early innate immune response. Type I interferon (IFN-I) is an integral part of this early innate response that primes several components of the adaptive immune response. Women are widely reported to respond better than men to tri- and quadrivalent influenza vaccines. Plasmacytoid dendritic cells (pDCs) are the primary cell type responsible for IFN-I production, and female pDCs produce more IFN-I than male pDCs since the upstream pattern recognition receptor Toll-like receptor 7 (TLR7) is encoded by X chromosome and is biallelically expressed by up to 30% of female immune cells. Additionally, the TLR7 promoter contains several putative androgen response elements, and androgens have been reported to suppress pDC IFN-I in vitro. Unexpectedly, therefore, we recently observed that male adolescents mount stronger antibody responses to the Pfizer BNT162b2 mRNA vaccine than female adolescents after controlling for natural SARS-CoV-2 infection. We here examined pDC behaviour in this same cohort to determine the impact of IFN-I on anti-spike and anti-receptor-binding domain IgG titres to BNT162b2. Through flow cytometry and least absolute shrinkage and selection operator (LASSO) modelling, we determined that serum-free testosterone was associated with reduced pDC IFN-I, but contrary to the well-described immunosuppressive role for androgens, the most bioactive androgen dihydrotestosterone was associated with increased IgG titres to BNT162b2. Also …
Authors
Oliver L Sampson,Cecilia Jay,Emily Adland,Anna Csala,Nicholas Lim,Stella M Ebbrecht,Lorna C Gilligan,Angela E Taylor,Sherley Sherafin George,Stephanie Longet,Lucy C Jones,Ellie Barnes,John Frater,Paul Klenerman,Susie Dunachie,Miles Carrol,James Hawley,Wiebke Arlt,Andreas Groll,Philip Goulder
Journal
Frontiers in immunology
Published Date
2024/2/28
Professor FAQs
What is paul klenerman's h-index at University of Oxford?
The h-index of paul klenerman has been 91 since 2020 and 136 in total.
What are paul klenerman's top articles?
The articles with the titles of
Immune responses and clinical outcomes after COVID-19 vaccination in patients with liver disease and liver transplant recipients
Multi-omics analysis reveals COVID-19 vaccine induced attenuation of inflammatory responses during breakthrough disease
MAIT cells play a role in liver tissue repair via growth factor secretion
The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA. 2.86
N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting
IL-5 antagonism reverses priming and activation of eosinophils in severe eosinophilic asthma
Obesity Differs from Diabetes Mellitus in Antibody and T Cell Responses Post COVID-19 Recovery
Gonadal androgens are associated with decreased type I interferon production by plasmacytoid dendritic cells and increased IgG titres to BNT162b2 following co-vaccination with …
...
are the top articles of paul klenerman at University of Oxford.
What are paul klenerman's research interests?
The research interests of paul klenerman are: Infection and Immunity
What is paul klenerman's total number of citations?
paul klenerman has 82,913 citations in total.