David Stuart
University of Oxford
H-index: 137
Europe-United Kingdom
Description
David Stuart, With an exceptional h-index of 137 and a recent h-index of 69 (since 2020), a distinguished researcher at University of Oxford, specializes in the field of Structural Biology, Virology, Crystallography, Synchrotron Radiation.
His recent articles reflect a diverse array of research interests and contributions to the field:
Cooperativity and induced oligomerization control the interaction of SARS-CoV-2 with its cellular receptor and patient-derived antibodies
Risk of SARS-CoV-2 reinfection during multiple Omicron variant waves in the UK general population
da Covid-19
The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA. 2.86
Structural comparison of typical and atypical E2 pestivirus glycoproteins
A delicate balance between antibody evasion and ACE2 affinity for Omicron BA. 2.75
Characterization of the rotavirus assembly pathway in situ using cryoelectron tomography
Experimental phasing opportunities for macromolecular crystallography at very long wavelengths
Professor Information
University | University of Oxford |
---|---|
Position | Diamond Light Source Insruct-ERIC |
Citations(all) | 71705 |
Citations(since 2020) | 23584 |
Cited By | 55291 |
hIndex(all) | 137 |
hIndex(since 2020) | 69 |
i10Index(all) | 463 |
i10Index(since 2020) | 306 |
University Profile Page | University of Oxford |
Research & Interests List
Structural Biology
Virology
Crystallography
Synchrotron Radiation
Top articles of David Stuart
Cooperativity and induced oligomerization control the interaction of SARS-CoV-2 with its cellular receptor and patient-derived antibodies
Successful viral entry requires efficient engagement of receptors on the host cell by proteins on the virus surface. Most proteins on the virus and cell surfaces are oligomeric, and therefore the underlying molecular interactions between them offer great scope for both the virus and the immune system to leverage the thermodynamic benefits of multivalent binding. Such an example is SARS-CoV-2 infection, involving a trimeric viral envelope spike protein that attaches to a dimeric angiotensin-converting enzyme 2 (ACE2) on host cells. Additionally, virus inhibition largely relies on antibodies that are effectively acting as dimers. While there is a growing appreciation of the importance of multivalency, its visualisation and quantification are challenging due to the resultant heterogeneity of the assembly products. Consequently, structural and biophysical characterisation almost exclusively require, or are interpreted within an …
Authors
Roi Asor,Anna Olerinyova,Sean A Burnap,Manish S Kushwah,Mario Hensen,Snežana Vasiljevic,Liu Chang,Wanwisa Dejnirattisa,Piyada Supasa,David I Stuart,Gavin R Screaton,Nicole Zitzmann,Justin LP Benesch,Weston B Struwe,Philipp Kukura
Journal
Biophysical Journal
Published Date
2024/2/8
Risk of SARS-CoV-2 reinfection during multiple Omicron variant waves in the UK general population
SARS-CoV-2 reinfections increased substantially after Omicron variants emerged. Large-scale community-based comparisons across multiple Omicron waves of reinfection characteristics, risk factors, and protection afforded by previous infection and vaccination, are limited. Here we studied ~45,000 reinfections from the UK’s national COVID-19 Infection Survey and quantified the risk of reinfection in multiple waves, including those driven by BA.1, BA.2, BA.4/5, and BQ.1/CH.1.1/XBB.1.5 variants. Reinfections were associated with lower viral load and lower percentages of self-reporting symptoms compared with first infections. Across multiple Omicron waves, estimated protection against reinfection was significantly higher in those previously infected with more recent than earlier variants, even at the same time from previous infection. Estimated protection against Omicron reinfections decreased over time from the …
Authors
Jia Wei,Nicole Stoesser,Philippa C Matthews,Tarnjit Khera,Owen Gethings,Ian Diamond,Ruth Studley,Nick Taylor,Tim EA Peto,A Sarah Walker,Koen B Pouwels,David W Eyre
Journal
Nature Communications
Published Date
2024/2/2
da Covid-19
COVID-19 | Medical Science Monitor H-Index 91 Scimago Lab powered by Scopus IF 2022 3.100 JCR Clarivate Analytics 14% Acceptance Rate call: ++1.631.470.9640 10:00 am - 02:00 pm EST Submit manuscript Authors login Logo Coronavirus / COVID-19 Home Page Submit Manuscript In Press CURRENT VOLUME: 30, 2024 Archives Volume 29, 2023 Volume 28, 2022 Volume 27, 2021 Volume 26, 2020 Volume 25, 2019 More... Volume 24, 2018 Volume 24, 2018 Supplement 1 Volume 23, 2017 Volume 22, 2016 Volume 21, 2015 Volume 20, 2014 Volume 19, 2013 Volume 18, 2012 Vol. 18 (12) 2012 Vol. 18 (11) 2012 Vol. 18 (10) 2012 Vol. 18 (9) 2012 Vol. 18 (8) 2012 Vol. 18 (7) 2012 Vol. 18 (6) 2012 Vol. 18 (5) 2012 Vol. 18 (4) 2012 Vol. 18 (3) 2012 Vol. 18 (2) 2012 Vol. 18 (1) 2012 Volume 17, 2011 Vol. 17 (12) 2011 Vol. 17 (11) 2011 Vol. 17 (10) 2011 Vol. 17 (9) 2011 Vol. 17 (8) 2011 Vol. 17 (7) 2011 Vol. …
Authors
WENHONG ZHANG
Journal
Med Sci Monit
Published Date
2024/2
The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA. 2.86
Under pressure from neutralising antibodies induced by vaccination or infection the SARS-CoV-2 spike gene has become a hotspot for evolutionary change, leading to the failure of all mAbs developed for clinical use. Most potent antibodies bind to the receptor binding domain which has become heavily mutated. Here we study responses to a conserved epitope in sub-domain-1 (SD1) of spike which have become more prominent because of mutational escape from antibodies directed to the receptor binding domain. Some SD1 reactive mAbs show potent and broad neutralization of SARS-CoV-2 variants. We structurally map the dominant SD1 epitope and provide a mechanism of action by blocking interaction with ACE2. Mutations in SD1 have not been sustained to date, but one, E554K, leads to escape from mAbs. This mutation has now emerged in several sublineages including BA.2.86, reflecting selection …
Authors
Daming Zhou,Piyada Supasa,Chang Liu,Aiste Dijokaite-Guraliuc,Helen ME Duyvesteyn,Muneeswaran Selvaraj,Alexander J Mentzer,Raksha Das,Wanwisa Dejnirattisai,Nigel Temperton,Paul Klenerman,Susanna J Dunachie,Elizabeth E Fry,Juthathip Mongkolsapaya,Jingshan Ren,David I Stuart,Gavin R Screaton
Journal
Nature Communications
Published Date
2024/3/28
Structural comparison of typical and atypical E2 pestivirus glycoproteins
Pestiviruses, within the family Flaviviridae, are economically important viruses of livestock. In recent years, new pestiviruses have been reported in domestic animals and non-cloven-hoofed animals. Among them, atypical porcine pestivirus (APPV) and Norway rat pestivirus (NRPV) have relatively little sequence conservation in their surface glycoprotein E2. Despite E2 being the main target for neutralizing antibodies and necessary for cell attachment and viral fusion, the mechanism of viral entry remains elusive. To gain further insights into the pestivirus E2 mechanism of action and to assess its diversity within the genus, we report X-ray structures of the pestivirus E2 proteins from APPV and NRPV. Despite the highly divergent structures, both are able to dimerize through their C-terminal domain and contain a solvent-exposed β-hairpin reported to be involved in host receptor binding. Functional analysis of this β …
Authors
Hazel Aitkenhead,Christiane Riedel,Nathan Cowieson,Hans Tillmann Rümenapf,David I Stuart,Kamel El Omari
Journal
Structure
Published Date
2024/3/7
A delicate balance between antibody evasion and ACE2 affinity for Omicron BA. 2.75
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have caused successive global waves of infection. These variants, with multiple mutations in the spike protein, are thought to facilitate escape from natural and vaccine-induced immunity and often increase in affinity for ACE2. The latest variant to cause concern is BA.2.75, identified in India where it is now the dominant strain, with evidence of wider dissemination. BA.2.75 is derived from BA.2 and contains four additional mutations in the receptor-binding domain (RBD). Here, we perform an antigenic and biophysical characterization of BA.2.75, revealing an interesting balance between humoral evasion and ACE2 receptor affinity. ACE2 affinity for BA.2.75 is increased 9-fold compared with BA.2; there is also evidence of escape of BA.2.75 from immune serum, particularly that induced by Delta infection, which may explain the rapid spread in …
Authors
Jiandong Huo,Aiste Dijokaite-Guraliuc,Chang Liu,Daming Zhou,Helen M Ginn,Raksha Das,Piyada Supasa,Muneeswaran Selvaraj,Rungtiwa Nutalai,Aekkachai Tuekprakhon,Helen ME Duyvesteyn,Alexander J Mentzer,Donal Skelly,Thomas G Ritter,Ali Amini,Sagida Bibi,Sandra Adele,Sile Ann Johnson,Neil G Paterson,Mark A Williams,David R Hall,Megan Plowright,Thomas AH Newman,Hailey Hornsby,Thushan I de Silva,Nigel Temperton,Paul Klenerman,Eleanor Barnes,Susanna J Dunachie,Andrew J Pollard,Teresa Lambe,Philip Goulder,Elizabeth E Fry,Juthathip Mongkolsapaya,Jingshan Ren,David I Stuart,Gavin R Screaton
Journal
Cell reports
Published Date
2023/1/31
Characterization of the rotavirus assembly pathway in situ using cryoelectron tomography
Rotavirus assembly is a complex process that involves the stepwise acquisition of protein layers in distinct intracellular locations to form the fully assembled particle. Understanding and visualization of the assembly process has been hampered by the inaccessibility of unstable intermediates. We characterize the assembly pathway of group A rotaviruses observed in situ within cryo-preserved infected cells through the use of cryoelectron tomography of cellular lamellae. Our findings demonstrate that the viral polymerase VP1 recruits viral genomes during particle assembly, as revealed by infecting with a conditionally lethal mutant. Additionally, pharmacological inhibition to arrest the transiently enveloped stage uncovered a unique conformation of the VP4 spike. Subtomogram averaging provided atomic models of four intermediate states, including a pre-packaging single-layered intermediate, the double-layered …
Authors
Pranav NM Shah,James B Gilchrist,Björn O Forsberg,Alister Burt,Andrew Howe,Shyamal Mosalaganti,William Wan,Julika Radecke,Yuriy Chaban,Geoff Sutton,David I Stuart,Mark Boyce
Journal
Cell host & microbe
Published Date
2023/4/12
Experimental phasing opportunities for macromolecular crystallography at very long wavelengths
Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range …
Authors
Kamel El Omari,Ramona Duman,Vitaliy Mykhaylyk,Christian M Orr,Merlyn Latimer-Smith,Graeme Winter,Vinay Grama,Feng Qu,Kiran Bountra,Hok Sau Kwong,Maria Romano,Rosana I Reis,Lutz Vogeley,Luca Vecchia,C David Owen,Sina Wittmann,Max Renner,Miki Senda,Naohiro Matsugaki,Yoshiaki Kawano,Thomas A Bowden,Isabel Moraes,Jonathan M Grimes,Erika J Mancini,Martin A Walsh,Cristiane R Guzzo,Raymond J Owens,E Yvonne Jones,David G Brown,Dave I Stuart,Konstantinos Beis,Armin Wagner
Journal
Communications Chemistry
Published Date
2023/10/12
Professor FAQs
What is David Stuart's h-index at University of Oxford?
The h-index of David Stuart has been 69 since 2020 and 137 in total.
What are David Stuart's top articles?
The articles with the titles of
Cooperativity and induced oligomerization control the interaction of SARS-CoV-2 with its cellular receptor and patient-derived antibodies
Risk of SARS-CoV-2 reinfection during multiple Omicron variant waves in the UK general population
da Covid-19
The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA. 2.86
Structural comparison of typical and atypical E2 pestivirus glycoproteins
A delicate balance between antibody evasion and ACE2 affinity for Omicron BA. 2.75
Characterization of the rotavirus assembly pathway in situ using cryoelectron tomography
Experimental phasing opportunities for macromolecular crystallography at very long wavelengths
...
are the top articles of David Stuart at University of Oxford.
What are David Stuart's research interests?
The research interests of David Stuart are: Structural Biology, Virology, Crystallography, Synchrotron Radiation
What is David Stuart's total number of citations?
David Stuart has 71,705 citations in total.
What are the co-authors of David Stuart?
The co-authors of David Stuart are E Yvonne Jones, Karl Harlos, Jingshan Ren, Jonathan Grimes, Raymond J Owens, Stephen Curry.